recombinational cloning Search Results


pez lv201 vector  (Genecopoeia)
94
Genecopoeia pez lv201 vector
Pez Lv201 Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinational+cloning/pm30026838-84-12-14?v=Genecopoeia
Average 94 stars, based on 1 article reviews
pez lv201 vector - by Bioz Stars, 2026-07
94/100 stars
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5 bromo 6 chloro 3 indolyl β d glucopyranoside  (Chem Impex International)
95
Chem Impex International 5 bromo 6 chloro 3 indolyl β d glucopyranoside
5 Bromo 6 Chloro 3 Indolyl β D Glucopyranoside, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinational+cloning/pmc08997480-51-20-22?v=Chem+Impex+International
Average 95 stars, based on 1 article reviews
5 bromo 6 chloro 3 indolyl β d glucopyranoside - by Bioz Stars, 2026-07
95/100 stars
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rad51b nm 133509 open reading frames  (OriGene)
94
OriGene rad51b nm 133509 open reading frames
( A ) ( Left panel ): dose–response curves of olaparib in colony formation assays in tetracycline-repressor (TR) expressing MDAMB436 cells with or without <t>RAD51B</t> complementation (MDAMB436-B-TR and MDAMB436-TR, respectively) and with (+BRCA1) or without (+LacZ) BRCA1 complementation. ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( B ) ( Left panel ) : dose–response curves of olaparib in colony formation assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( C ) ( Left panel ): dose–response curves of olaparib in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+C64R, +L1407P, +R1699Q). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. Data for BRCA1 hypomorphs with deletions in the RING (RING-less, M48 START) or BRCT (BRCT-less) domains are included for comparative purposes. ( D ) ( Left panel ): dose–response curves of carboplatin in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ) or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less, +CC-mutant). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of carboplatin for each cell line. ( E ) Western blot of immunoprecipitation experiments in MDAMB436-B-TR cells expressing full-length (+FL) or ∆exon11 BRCA1 and exposed to different doxycycline doses. GAPDH was used as a loading control. I = input; F = FLAG immunoprecipitation. ( F ) Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib at the different doses of doxycycline used. All data are from at least 3 biological replicates. Statistical analysis performed using One-Way ANOVA with Holm–Sidak multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. CFU = colony-forming units. Dotted lines in the olaparib IC50 graphs represent the minimal free concentration of olaparib (approximately 300 nM) in the plasma of patients on the established monotherapy dose of 300 mg twice daily. The original western blot figures can be found in .
Rad51b Nm 133509 Open Reading Frames, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinational+cloning/pmc12838977-59-7-19?v=OriGene
Average 94 stars, based on 1 article reviews
rad51b nm 133509 open reading frames - by Bioz Stars, 2026-07
94/100 stars
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pez m06 eukaryotic expression vector  (Genecopoeia)
94
Genecopoeia pez m06 eukaryotic expression vector
( A ) ( Left panel ): dose–response curves of olaparib in colony formation assays in tetracycline-repressor (TR) expressing MDAMB436 cells with or without <t>RAD51B</t> complementation (MDAMB436-B-TR and MDAMB436-TR, respectively) and with (+BRCA1) or without (+LacZ) BRCA1 complementation. ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( B ) ( Left panel ) : dose–response curves of olaparib in colony formation assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( C ) ( Left panel ): dose–response curves of olaparib in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+C64R, +L1407P, +R1699Q). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. Data for BRCA1 hypomorphs with deletions in the RING (RING-less, M48 START) or BRCT (BRCT-less) domains are included for comparative purposes. ( D ) ( Left panel ): dose–response curves of carboplatin in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ) or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less, +CC-mutant). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of carboplatin for each cell line. ( E ) Western blot of immunoprecipitation experiments in MDAMB436-B-TR cells expressing full-length (+FL) or ∆exon11 BRCA1 and exposed to different doxycycline doses. GAPDH was used as a loading control. I = input; F = FLAG immunoprecipitation. ( F ) Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib at the different doses of doxycycline used. All data are from at least 3 biological replicates. Statistical analysis performed using One-Way ANOVA with Holm–Sidak multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. CFU = colony-forming units. Dotted lines in the olaparib IC50 graphs represent the minimal free concentration of olaparib (approximately 300 nM) in the plasma of patients on the established monotherapy dose of 300 mg twice daily. The original western blot figures can be found in .
Pez M06 Eukaryotic Expression Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinational+cloning/pmc03750158-122-14-19?v=Genecopoeia
Average 94 stars, based on 1 article reviews
pez m06 eukaryotic expression vector - by Bioz Stars, 2026-07
94/100 stars
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cag promoter  (Genecopoeia)
94
Genecopoeia cag promoter
( A ) ( Left panel ): dose–response curves of olaparib in colony formation assays in tetracycline-repressor (TR) expressing MDAMB436 cells with or without <t>RAD51B</t> complementation (MDAMB436-B-TR and MDAMB436-TR, respectively) and with (+BRCA1) or without (+LacZ) BRCA1 complementation. ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( B ) ( Left panel ) : dose–response curves of olaparib in colony formation assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( C ) ( Left panel ): dose–response curves of olaparib in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+C64R, +L1407P, +R1699Q). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. Data for BRCA1 hypomorphs with deletions in the RING (RING-less, M48 START) or BRCT (BRCT-less) domains are included for comparative purposes. ( D ) ( Left panel ): dose–response curves of carboplatin in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ) or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less, +CC-mutant). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of carboplatin for each cell line. ( E ) Western blot of immunoprecipitation experiments in MDAMB436-B-TR cells expressing full-length (+FL) or ∆exon11 BRCA1 and exposed to different doxycycline doses. GAPDH was used as a loading control. I = input; F = FLAG immunoprecipitation. ( F ) Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib at the different doses of doxycycline used. All data are from at least 3 biological replicates. Statistical analysis performed using One-Way ANOVA with Holm–Sidak multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. CFU = colony-forming units. Dotted lines in the olaparib IC50 graphs represent the minimal free concentration of olaparib (approximately 300 nM) in the plasma of patients on the established monotherapy dose of 300 mg twice daily. The original western blot figures can be found in .
Cag Promoter, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinational+cloning/pm41781491-84-25-27?v=Genecopoeia
Average 94 stars, based on 1 article reviews
cag promoter - by Bioz Stars, 2026-07
94/100 stars
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expression vector pez m02 through recombination  (Genecopoeia)
94
Genecopoeia expression vector pez m02 through recombination
( A ) ( Left panel ): dose–response curves of olaparib in colony formation assays in tetracycline-repressor (TR) expressing MDAMB436 cells with or without <t>RAD51B</t> complementation (MDAMB436-B-TR and MDAMB436-TR, respectively) and with (+BRCA1) or without (+LacZ) BRCA1 complementation. ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( B ) ( Left panel ) : dose–response curves of olaparib in colony formation assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( C ) ( Left panel ): dose–response curves of olaparib in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+C64R, +L1407P, +R1699Q). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. Data for BRCA1 hypomorphs with deletions in the RING (RING-less, M48 START) or BRCT (BRCT-less) domains are included for comparative purposes. ( D ) ( Left panel ): dose–response curves of carboplatin in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ) or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less, +CC-mutant). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of carboplatin for each cell line. ( E ) Western blot of immunoprecipitation experiments in MDAMB436-B-TR cells expressing full-length (+FL) or ∆exon11 BRCA1 and exposed to different doxycycline doses. GAPDH was used as a loading control. I = input; F = FLAG immunoprecipitation. ( F ) Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib at the different doses of doxycycline used. All data are from at least 3 biological replicates. Statistical analysis performed using One-Way ANOVA with Holm–Sidak multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. CFU = colony-forming units. Dotted lines in the olaparib IC50 graphs represent the minimal free concentration of olaparib (approximately 300 nM) in the plasma of patients on the established monotherapy dose of 300 mg twice daily. The original western blot figures can be found in .
Expression Vector Pez M02 Through Recombination, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinational+cloning/pmc04360034-198-24-29?v=Genecopoeia
Average 94 stars, based on 1 article reviews
expression vector pez m02 through recombination - by Bioz Stars, 2026-07
94/100 stars
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ezshuttle recombination cloning expression vector pez lv199  (Genecopoeia)
94
Genecopoeia ezshuttle recombination cloning expression vector pez lv199
( A ) ( Left panel ): dose–response curves of olaparib in colony formation assays in tetracycline-repressor (TR) expressing MDAMB436 cells with or without <t>RAD51B</t> complementation (MDAMB436-B-TR and MDAMB436-TR, respectively) and with (+BRCA1) or without (+LacZ) BRCA1 complementation. ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( B ) ( Left panel ) : dose–response curves of olaparib in colony formation assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( C ) ( Left panel ): dose–response curves of olaparib in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+C64R, +L1407P, +R1699Q). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. Data for BRCA1 hypomorphs with deletions in the RING (RING-less, M48 START) or BRCT (BRCT-less) domains are included for comparative purposes. ( D ) ( Left panel ): dose–response curves of carboplatin in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ) or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less, +CC-mutant). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of carboplatin for each cell line. ( E ) Western blot of immunoprecipitation experiments in MDAMB436-B-TR cells expressing full-length (+FL) or ∆exon11 BRCA1 and exposed to different doxycycline doses. GAPDH was used as a loading control. I = input; F = FLAG immunoprecipitation. ( F ) Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib at the different doses of doxycycline used. All data are from at least 3 biological replicates. Statistical analysis performed using One-Way ANOVA with Holm–Sidak multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. CFU = colony-forming units. Dotted lines in the olaparib IC50 graphs represent the minimal free concentration of olaparib (approximately 300 nM) in the plasma of patients on the established monotherapy dose of 300 mg twice daily. The original western blot figures can be found in .
Ezshuttle Recombination Cloning Expression Vector Pez Lv199, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinational+cloning/pm30332648-264-9-15?v=Genecopoeia
Average 94 stars, based on 1 article reviews
ezshuttle recombination cloning expression vector pez lv199 - by Bioz Stars, 2026-07
94/100 stars
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pez m68 vector  (Genecopoeia)
94
Genecopoeia pez m68 vector
( A ) ( Left panel ): dose–response curves of olaparib in colony formation assays in tetracycline-repressor (TR) expressing MDAMB436 cells with or without <t>RAD51B</t> complementation (MDAMB436-B-TR and MDAMB436-TR, respectively) and with (+BRCA1) or without (+LacZ) BRCA1 complementation. ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( B ) ( Left panel ) : dose–response curves of olaparib in colony formation assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( C ) ( Left panel ): dose–response curves of olaparib in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+C64R, +L1407P, +R1699Q). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. Data for BRCA1 hypomorphs with deletions in the RING (RING-less, M48 START) or BRCT (BRCT-less) domains are included for comparative purposes. ( D ) ( Left panel ): dose–response curves of carboplatin in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ) or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less, +CC-mutant). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of carboplatin for each cell line. ( E ) Western blot of immunoprecipitation experiments in MDAMB436-B-TR cells expressing full-length (+FL) or ∆exon11 BRCA1 and exposed to different doxycycline doses. GAPDH was used as a loading control. I = input; F = FLAG immunoprecipitation. ( F ) Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib at the different doses of doxycycline used. All data are from at least 3 biological replicates. Statistical analysis performed using One-Way ANOVA with Holm–Sidak multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. CFU = colony-forming units. Dotted lines in the olaparib IC50 graphs represent the minimal free concentration of olaparib (approximately 300 nM) in the plasma of patients on the established monotherapy dose of 300 mg twice daily. The original western blot figures can be found in .
Pez M68 Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinational+cloning/pmc07244012-167-20-22?v=Genecopoeia
Average 94 stars, based on 1 article reviews
pez m68 vector - by Bioz Stars, 2026-07
94/100 stars
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expression vector pez m67  (Genecopoeia)
94
Genecopoeia expression vector pez m67
( A ) ( Left panel ): dose–response curves of olaparib in colony formation assays in tetracycline-repressor (TR) expressing MDAMB436 cells with or without <t>RAD51B</t> complementation (MDAMB436-B-TR and MDAMB436-TR, respectively) and with (+BRCA1) or without (+LacZ) BRCA1 complementation. ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( B ) ( Left panel ) : dose–response curves of olaparib in colony formation assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( C ) ( Left panel ): dose–response curves of olaparib in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+C64R, +L1407P, +R1699Q). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. Data for BRCA1 hypomorphs with deletions in the RING (RING-less, M48 START) or BRCT (BRCT-less) domains are included for comparative purposes. ( D ) ( Left panel ): dose–response curves of carboplatin in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ) or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less, +CC-mutant). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of carboplatin for each cell line. ( E ) Western blot of immunoprecipitation experiments in MDAMB436-B-TR cells expressing full-length (+FL) or ∆exon11 BRCA1 and exposed to different doxycycline doses. GAPDH was used as a loading control. I = input; F = FLAG immunoprecipitation. ( F ) Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib at the different doses of doxycycline used. All data are from at least 3 biological replicates. Statistical analysis performed using One-Way ANOVA with Holm–Sidak multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. CFU = colony-forming units. Dotted lines in the olaparib IC50 graphs represent the minimal free concentration of olaparib (approximately 300 nM) in the plasma of patients on the established monotherapy dose of 300 mg twice daily. The original western blot figures can be found in .
Expression Vector Pez M67, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinational+cloning/pm32267274-32-5-11?v=Genecopoeia
Average 94 stars, based on 1 article reviews
expression vector pez m67 - by Bioz Stars, 2026-07
94/100 stars
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pez lv195 vector  (Genecopoeia)
94
Genecopoeia pez lv195 vector
( A ) ( Left panel ): dose–response curves of olaparib in colony formation assays in tetracycline-repressor (TR) expressing MDAMB436 cells with or without <t>RAD51B</t> complementation (MDAMB436-B-TR and MDAMB436-TR, respectively) and with (+BRCA1) or without (+LacZ) BRCA1 complementation. ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( B ) ( Left panel ) : dose–response curves of olaparib in colony formation assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( C ) ( Left panel ): dose–response curves of olaparib in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+C64R, +L1407P, +R1699Q). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. Data for BRCA1 hypomorphs with deletions in the RING (RING-less, M48 START) or BRCT (BRCT-less) domains are included for comparative purposes. ( D ) ( Left panel ): dose–response curves of carboplatin in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ) or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less, +CC-mutant). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of carboplatin for each cell line. ( E ) Western blot of immunoprecipitation experiments in MDAMB436-B-TR cells expressing full-length (+FL) or ∆exon11 BRCA1 and exposed to different doxycycline doses. GAPDH was used as a loading control. I = input; F = FLAG immunoprecipitation. ( F ) Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib at the different doses of doxycycline used. All data are from at least 3 biological replicates. Statistical analysis performed using One-Way ANOVA with Holm–Sidak multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. CFU = colony-forming units. Dotted lines in the olaparib IC50 graphs represent the minimal free concentration of olaparib (approximately 300 nM) in the plasma of patients on the established monotherapy dose of 300 mg twice daily. The original western blot figures can be found in .
Pez Lv195 Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinational+cloning/pmc08431472-149-54-56?v=Genecopoeia
Average 94 stars, based on 1 article reviews
pez lv195 vector - by Bioz Stars, 2026-07
94/100 stars
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recombinant escherichia coli  (Cusabio)
93
Cusabio recombinant escherichia coli
( A ) ( Left panel ): dose–response curves of olaparib in colony formation assays in tetracycline-repressor (TR) expressing MDAMB436 cells with or without <t>RAD51B</t> complementation (MDAMB436-B-TR and MDAMB436-TR, respectively) and with (+BRCA1) or without (+LacZ) BRCA1 complementation. ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( B ) ( Left panel ) : dose–response curves of olaparib in colony formation assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( C ) ( Left panel ): dose–response curves of olaparib in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+C64R, +L1407P, +R1699Q). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. Data for BRCA1 hypomorphs with deletions in the RING (RING-less, M48 START) or BRCT (BRCT-less) domains are included for comparative purposes. ( D ) ( Left panel ): dose–response curves of carboplatin in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ) or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less, +CC-mutant). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of carboplatin for each cell line. ( E ) Western blot of immunoprecipitation experiments in MDAMB436-B-TR cells expressing full-length (+FL) or ∆exon11 BRCA1 and exposed to different doxycycline doses. GAPDH was used as a loading control. I = input; F = FLAG immunoprecipitation. ( F ) Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib at the different doses of doxycycline used. All data are from at least 3 biological replicates. Statistical analysis performed using One-Way ANOVA with Holm–Sidak multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. CFU = colony-forming units. Dotted lines in the olaparib IC50 graphs represent the minimal free concentration of olaparib (approximately 300 nM) in the plasma of patients on the established monotherapy dose of 300 mg twice daily. The original western blot figures can be found in .
Recombinant Escherichia Coli, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinational+cloning/pmc12718106-43-10-19?v=Cusabio
Average 93 stars, based on 1 article reviews
recombinant escherichia coli - by Bioz Stars, 2026-07
93/100 stars
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expression vector pez m09  (Genecopoeia)
94
Genecopoeia expression vector pez m09
( A ) ( Left panel ): dose–response curves of olaparib in colony formation assays in tetracycline-repressor (TR) expressing MDAMB436 cells with or without <t>RAD51B</t> complementation (MDAMB436-B-TR and MDAMB436-TR, respectively) and with (+BRCA1) or without (+LacZ) BRCA1 complementation. ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( B ) ( Left panel ) : dose–response curves of olaparib in colony formation assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( C ) ( Left panel ): dose–response curves of olaparib in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+C64R, +L1407P, +R1699Q). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. Data for BRCA1 hypomorphs with deletions in the RING (RING-less, M48 START) or BRCT (BRCT-less) domains are included for comparative purposes. ( D ) ( Left panel ): dose–response curves of carboplatin in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ) or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less, +CC-mutant). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of carboplatin for each cell line. ( E ) Western blot of immunoprecipitation experiments in MDAMB436-B-TR cells expressing full-length (+FL) or ∆exon11 BRCA1 and exposed to different doxycycline doses. GAPDH was used as a loading control. I = input; F = FLAG immunoprecipitation. ( F ) Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib at the different doses of doxycycline used. All data are from at least 3 biological replicates. Statistical analysis performed using One-Way ANOVA with Holm–Sidak multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. CFU = colony-forming units. Dotted lines in the olaparib IC50 graphs represent the minimal free concentration of olaparib (approximately 300 nM) in the plasma of patients on the established monotherapy dose of 300 mg twice daily. The original western blot figures can be found in .
Expression Vector Pez M09, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) ( Left panel ): dose–response curves of olaparib in colony formation assays in tetracycline-repressor (TR) expressing MDAMB436 cells with or without RAD51B complementation (MDAMB436-B-TR and MDAMB436-TR, respectively) and with (+BRCA1) or without (+LacZ) BRCA1 complementation. ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( B ) ( Left panel ) : dose–response curves of olaparib in colony formation assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( C ) ( Left panel ): dose–response curves of olaparib in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+C64R, +L1407P, +R1699Q). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. Data for BRCA1 hypomorphs with deletions in the RING (RING-less, M48 START) or BRCT (BRCT-less) domains are included for comparative purposes. ( D ) ( Left panel ): dose–response curves of carboplatin in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ) or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less, +CC-mutant). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of carboplatin for each cell line. ( E ) Western blot of immunoprecipitation experiments in MDAMB436-B-TR cells expressing full-length (+FL) or ∆exon11 BRCA1 and exposed to different doxycycline doses. GAPDH was used as a loading control. I = input; F = FLAG immunoprecipitation. ( F ) Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib at the different doses of doxycycline used. All data are from at least 3 biological replicates. Statistical analysis performed using One-Way ANOVA with Holm–Sidak multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. CFU = colony-forming units. Dotted lines in the olaparib IC50 graphs represent the minimal free concentration of olaparib (approximately 300 nM) in the plasma of patients on the established monotherapy dose of 300 mg twice daily. The original western blot figures can be found in .

Journal: Cancers

Article Title: An Inducible BRCA1 Expression System with In Vivo Applicability Uncovers Activity of the Combination of ATR and PARP Inhibitors to Overcome Therapy Resistance

doi: 10.3390/cancers18020309

Figure Lengend Snippet: ( A ) ( Left panel ): dose–response curves of olaparib in colony formation assays in tetracycline-repressor (TR) expressing MDAMB436 cells with or without RAD51B complementation (MDAMB436-B-TR and MDAMB436-TR, respectively) and with (+BRCA1) or without (+LacZ) BRCA1 complementation. ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( B ) ( Left panel ) : dose–response curves of olaparib in colony formation assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. ( C ) ( Left panel ): dose–response curves of olaparib in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ), or different BRCA1 hypomorphs (+C64R, +L1407P, +R1699Q). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib for each cell line. Data for BRCA1 hypomorphs with deletions in the RING (RING-less, M48 START) or BRCT (BRCT-less) domains are included for comparative purposes. ( D ) ( Left panel ): dose–response curves of carboplatin in survival assays in MDAMB436-B-TR cells expressing full-length BRCA1 (+FL), no BRCA1 (+LacZ) or different BRCA1 hypomorphs (+RING-less, +∆exon11, +BRCT-less, +CC-mutant). ( Right panel ): Logarithmic half-maximal inhibitory concentration (LogIC50) of carboplatin for each cell line. ( E ) Western blot of immunoprecipitation experiments in MDAMB436-B-TR cells expressing full-length (+FL) or ∆exon11 BRCA1 and exposed to different doxycycline doses. GAPDH was used as a loading control. I = input; F = FLAG immunoprecipitation. ( F ) Logarithmic half-maximal inhibitory concentration (LogIC50) of olaparib at the different doses of doxycycline used. All data are from at least 3 biological replicates. Statistical analysis performed using One-Way ANOVA with Holm–Sidak multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. CFU = colony-forming units. Dotted lines in the olaparib IC50 graphs represent the minimal free concentration of olaparib (approximately 300 nM) in the plasma of patients on the established monotherapy dose of 300 mg twice daily. The original western blot figures can be found in .

Article Snippet: Plasmids containing the human BRCA1 (NM_007294) and RAD51B (NM_133509) open reading frames (ORFs) were obtained from Genscript (Ohu18572D) and OriGene (RC206457L3), respectively.

Techniques: Expressing, Concentration Assay, Mutagenesis, Western Blot, Immunoprecipitation, Control, Clinical Proteomics